Determination of Atenolol and Hydrochlorothiazide in Pharmaceutical Preparation Using RP-HPLC

Authors

  • Saad Antakli Faculty of Science | University of Aleppo | Syria
  • Leon Nejem Faculty of Science | University of Aleppo | Syria
  • Moustafa Alabo Joumaa Faculty of Science | University of Aleppo | Syria

DOI:

https://doi.org/10.26389/AJSRP.Y261021

Keywords:

HPLC, Atenolol, Hydrochlorothiazide

Abstract

A (Reversed Phase High Performance Liquid Chromatography) (RP-HPLC) method was Development for the determination of Atenolol (ATE) and Hydrochlorothiazide (HCT) in tablets formulations. Isocratic Elution Chromatography analysis was achieved with (methanol: phosphoric acid) (pH = 4) in ratio of 70:30 (v/v) as mobile phase, the analytical is done by C18 column and UV detector at 220 nm, pump flow rate was 1.2 mL/min and sample injection volume 20 μL.

The proposed method was successfully applied to analysis individual or mixture of (ATE) and (HCT) in Syrian trademark drugs. All studied samples showed that the drug levels were conformed to British Pharmacopeia. Finally, the method is simple, selective and suitable for routine quality control analysis.

Author Biographies

  • Saad Antakli, Faculty of Science | University of Aleppo | Syria

    Faculty of Science | University of Aleppo | Syria

  • Leon Nejem, Faculty of Science | University of Aleppo | Syria

    Faculty of Science | University of Aleppo | Syria

  • Moustafa Alabo Joumaa, Faculty of Science | University of Aleppo | Syria

    Faculty of Science | University of Aleppo | Syria

References

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Published

2022-09-28

Issue

Vol. 6 No. 3 (2022)

Section

Content

License

Copyright (c) 2022 Arab Journal of Sciences & Research Publishing - AJSRP

How to Cite

Antakli, S., Nejem, L., & Joumaa, M. A. (2022). Determination of Atenolol and Hydrochlorothiazide in Pharmaceutical Preparation Using RP-HPLC. Journal of Natural Sciences, Life and Applied Sciences, 6(3), 144-152. https://doi.org/10.26389/AJSRP.Y261021