Characterization of melanoidin formed from glucose with alanine  by Fluorescence spectroscopy and redox chemistry in vitro cells

Authors

  • Ghassan Faisal Mohsin General Directorate Vocational Education | Ministry of Education | Iraq
  • Abdulridha Ati Jaafar Faculty of Agriculture | University of Misan | Iraq

DOI:

https://doi.org/10.26389/AJSRP.G120919

Keywords:

melanoidin, fluorescence spectroscopy, hydrogen peroxide, cells

Abstract

Possible identify and describe a polymer formed from Glc with Ala by Fluorescence spectroscopy. Melanoidins before dialysis was higher than those of melanoidin after dialysis. Time‐resolved fluorescence has been applied in the characterization of melanoidin and was observed. Melanoidins show a decrease in the absorption after the addition of hydrogen peroxide when the concentrations of H2O2 from 100 - 200 μl are used. However, the data suggests that the melanoidin did not penetrate into the cells (CHO-K1) after 1 and 2 hours, but it can be observed after 24 hours.

Abbreviation: TRES, time-resolved emission spectra ; Glc, Glucose ; Ala, Alanine ; YFP, Yellow fluorescence protein ; GFP, green fluorescence protein ; CHO, Chinese hamster ovary

Author Biographies

  • Ghassan Faisal Mohsin, General Directorate Vocational Education | Ministry of Education | Iraq

    General Directorate Vocational Education | Ministry of Education | Iraq

  • Abdulridha Ati Jaafar, Faculty of Agriculture | University of Misan | Iraq

    Faculty of Agriculture | University of Misan | Iraq

References

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Published

2020-03-30

Issue

Vol. 4 No. 1 (2020)

Section

Content

License

Copyright (c) 2020 المجلة العربية للعلوم ونشر الأبحاث

How to Cite

Mohsin, G. F., & Jaafar, A. A. (2020). Characterization of melanoidin formed from glucose with alanine  by Fluorescence spectroscopy and redox chemistry in vitro cells. Journal of Natural Sciences, Life and Applied Sciences, 4(1), 29-17. https://doi.org/10.26389/AJSRP.G120919